@article {72, title = {Two-photon polymerization 3D-printing of micro-scale neuronal cell culture devices}, journal = {Journal of Visualized Experiments}, volume = {208}, year = {2024}, pages = {e66142}, abstract = {

Neuronal cultures have been a reference experimental model for several decades. However, 3D cell arrangement, spatial constraints on neurite outgrowth, and realistic synaptic connectivity are missing. The latter limits the study of structure and function in the context of compartmentalization and diminishes the significance of cultures in neuroscience. Approximating ex vivo the structured anatomical arrangement of synaptic connectivity is not trivial, despite being key for the emergence of rhythms, synaptic plasticity, and ultimately, brain pathophysiology. Here, two-photon polymerization (2PP) is employed as a 3D-printing technique, enabling the rapid fabrication of polymeric cell culture devices using polydimethyl-siloxane (PDMS) at the micrometer scale. Compared to conventional replica molding techniques based on microphotolitography, 2PP micro-scale printing enables rapid and affordable turnaround of prototypes. This protocol illustrates the design and fabrication of PDMS-based microfluidic devices aimed at culturing modular neuronal networks. As a proof-of-principle, a two-chamber device is presented to physically constrain connectivity. Specifically, an asymmetric axonal outgrowth during ex vivo development is favored and allowed to be directed from one chamber to the other. In order to probe the functional consequences of unidirectional synaptic interactions, commercial microelectrode arrays are chosen to monitor the bioelectrical activity of interconnected neuronal modules. Here, methods to 1) fabricate molds with micrometer precision and 2) perform in vitro multisite extracellular recordings in rat cortical neuronal cultures are illustrated. By decreasing costs and future widespread accessibility of 2PP 3D-printing, this method will become more and more relevant across research labs worldwide. Especially in neurotechnology and high-throughput neural data recording, the ease and rapidity of prototyping simplified in vitro models will improve experimental control and theoretical understanding of in vivo large-scale neural systems.

}, doi = {10.3791/66142}, author = {Ali Hosseini and Giovanni Noselli and Michele Giugliano} } @article {67, title = {Transient shape morphing of active gel plates: Geometry and physics}, journal = {Soft Matter}, volume = {18}, year = {2022}, pages = {5867-5876}, chapter = {5867}, abstract = {

The control of shape in active structures is a key problem for the realization of smart sensors and actuators, which often draw inspiration from natural systems. In this context, slender structures, such as thin plates, have been studied as a relevant example of shape morphing systems where curvature is generated by in-plane incompatibilities. In particular, in hydrogel plates these incompatibilities can be programmed at fabrication time, such that a target configuration is attained at equilibrium upon swelling or shrinking. While these aspects have been examined in detail, understanding the transient morphing of such active structures deserves further investigation. In this study, we develop a geometrical model for the transient shaping of thin hydrogel plates by extending the theory of non-Euclidean plates. We validate the proposed model using experiments on gel samples that are programmed to reach axisymmetric equilibrium shapes. Interestingly, our experiments show the emergence of non-axisymmetric shapes for early times, as a consequence of boundary layer effects induced by solvent transport. We rationalize these observations using numerical simulations based on a detailed poroelastic model. Overall, this work highlights the limitations of purely geometrical models and the importance of transient, reduced theories for morphing plates that account for the coupled physics driving the evolution of shape. Computational approaches employing these theories will allow to achieve accurate control on the morphing dynamics and ultimately advance 4D printing technologies.\ 

}, keywords = {active materials, hydrogel plates, Transient morphing}, doi = {10.1039/D2SM00669C}, author = {Valentina Damioli and Erik Zorzin and Antonio DeSimone and Giovanni Noselli and Alessandro Lucantonio} } @article {61, title = {A theoretical study on the transient morphing of linear poroelastic plates}, journal = {Journal of Applied Mechanics}, volume = {88}, year = {2021}, pages = {031008}, abstract = {

Based on their shape-shifting capabilities, soft active materials have enabled new possibilities for the engineering of sensing and actuation devices. While the relation between active strains and emergent equilibrium shapes has been fully characterized, the transient morphing of thin structures is a rather unexplored topic. Here, we focus on polymer gel plates and derive a reduced linear model to study their time-dependent response to changes in the fluid environment. We show that independent control of stretching and bending deformations in stress-free conditions allows to realize spherical shapes with prescribed geometry of the mid-plane. Furthermore, we demonstrate that tensile (compressive) membrane stresses delay (accelerate) swelling-induced shape transitions compared to the stress-free evolution. We believe that these effects should be considered for the accurate design of smart systems and may contribute to explain the complexity of natural shapes.\ 

}, keywords = {Dimensional reduction, Polymer gel plates, Sensing and actuation devices, Shape-shifting structures, Transient morphing}, doi = {10.1115/1.4048806}, author = {Andrini, Dario and Lucantonio, Alessandro and Noselli, Giovanni} } @article {60, title = {A theoretical study on the transient morphing of linear poroelastic plates}, journal = {Journal of Applied Mechanics}, volume = {In press}, year = {2020}, abstract = {

Based on their shape-shifting capabilities, soft active materials have enabled new possibilities for the engineering of sensing and actuation devices. While the relation between active strains and emergent equilibrium shapes has been fully characterized, the transient morphing of thin structures is a rather unexplored topic. Here, we focus on polymer gel plates and derive a reduced linear model to study their time-dependent response to changes in the fluid environment. We show that independent control of stretching and bending deformations in stress-free conditions allows to realize spherical shapes with prescribed geometry of the mid-plane. Further, we demonstrate that tensile (compressive) membrane stresses delay (accelerate) swelling-induced shape transitions, as compared to the stress-free evolution. We believe that these effects should be considered for the accurate design of smart systems and may contribute to explain the complexity of natural shapes.\ 

}, keywords = {Dimensional reduction, Polymer gel plates, Sensing and actuation devices, Shape-shifting structures, Transient morphing}, doi = {10.1115/1.4048806}, author = {Dario Andrini and Alessandro Lucantonio and Giovanni Noselli} }